2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Ihe ndị a na-ejikarị eme ihe na usoro nhazi nke nkesa oke molekul: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Ngwa na akụrụngwa
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
N'ozuzu, oke amino acid dị na ngwaahịa Sustar dị elu karịa nke dị na ngwaahịa Zinpro.
Nkebi nke 8 Mmetụta ojiji
Mmetụta nke isi mmalite dị iche iche nke mineral na arụmọrụ mmepụta na ịdị mma nke àkwá nke ọkụkọ na-etinye n'oge mgbụsị akwụkwọ.
Usoro Mmepụta
Teknụzụ chelation ezubere iche
Teknụzụ emulsification shear
Teknụzụ ịgba nrụgide na ihicha
Teknụzụ maka ime ka mmiri dị jụụ na friji
Teknụzụ njikwa gburugburu ebe obibi dị elu
Mgbakwunye A: Ụzọ maka ikpebi nkesa oke molekul nke peptides
Nkwenye nke ọkọlọtọ: GB/T 22492-2008
1 Ụkpụrụ Ule:
E ji usoro nzacha gel dị elu chọpụta ya. Nke ahụ pụtara, site na iji ihe nzacha porous dị ka usoro kwụ ọtọ, dabere na ọdịiche dị na nha oke molekul nke ihe nlele maka nkewa, nke achọpụtara na njikọ peptide nke ogologo oge nnabata ultraviolet nke 220nm, site na iji ngwanrọ nhazi data raara onwe ya nye maka mkpebi nke nkesa oke molekul site na iji gel filtration chromatography (ya bụ, ngwanrọ GPC), e mepụtara chromatograms na data ha, gbakọọ iji nweta nha oke molekul nke peptide soybean na oke nkesa.
2. Ihe ndị na-eme ka ihe dịghachi ndụ
Mmiri nnwale ahụ kwesịrị iru nkọwapụta nke mmiri nke abụọ na GB/T6682, ojiji nke ihe ndị na-eme ihe, ma e wezụga ihe pụrụ iche, dị ọcha n'ihe gbasara nyocha.
2.1 Ihe ndị na-eme ka mmeghachi omume dị na ya gụnyere acetonitrile (dị ọcha n'ụdị chromatographic), trifluoroacetic acid (dị ọcha n'ụdị chromatographic),
2.2 Ihe ndị a na-ejikarị eme ihe na usoro nhazi nke nkesa oke molekul: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Ngwa na akụrụngwa
3.1 Igwe Chromatograph Mmiri Nwere Ọrụ Dị Elu (HPLC): ebe ọrụ chromatographic ma ọ bụ ihe njikọta nwere ihe nchọpụta UV na ngwanrọ nhazi data GPC.
3.2 Ngwa nzacha na mwepụ nke oghere ikuku nke na-agagharị agagharị.
3.3 Nha eletrọniki: uru gụchara 0.000 1g.
Usoro ọrụ 4
4.1 Ọnọdụ Chromatographic na nnwale mgbanwe sistemụ (ọnọdụ ntụaka)
- 4.1.1 Kọlụm Chromatographic: TSKgelG2000swxl300 mm × 7.8 mm (dayameta dị n'ime) ma ọ bụ kọlụm gel ndị ọzọ nke otu ụdị nwere arụmọrụ yiri nke ahụ dabara adaba maka mkpebi nke protein na peptides.
- 4.1.2 Usoro mkpagharị: Acetonitrile + mmiri + trifluoroacetic acid = 20 + 80 + 0.1.
- 4.1.3 Ogologo ogologo nchọpụta: 220 nm.
- 4.1.4 Ọkwa mmiri: 0.5 mL/min.
- 4.1.5 Oge nchọpụta: nkeji iri atọ.
- 4.1.6 Olu ihe nlele nke ọgwụ: 20μL.
- 4.1.7 Okpomọkụ kọlụm: okpomọkụ ụlọ.
- 4.1.8 Iji mee ka sistemụ chromatographic mezuo ihe achọrọ maka nchọpụta, e kwuru na n'okpuru ọnọdụ chromatographic dị n'elu, arụmọrụ nke kọlụm gel chromatographic, ya bụ, ọnụọgụ nke efere echiche (N), erughị 10000 gbakọrọ dabere na elu nke ọkọlọtọ tripeptide (Glycine-Glycine-Glycine).
- 4.2 Mmepụta nke usoro ọkọlọtọ nke oke molekul
- E kwadebere ihe ngwọta peptide molekul dị iche iche dị n'elu nwere oke mkpokọta nke 1 mg / mL site na nhazi usoro mkpanaka, gwakọta ya na oke ụfọdụ, wee wụsa ya site na akpụkpọ ahụ organic nke nwere oke oghere nke 0.2 μm ~ 0.5 μm wee gbanye ya n'ime ihe nlele ahụ, wee nweta chromatograms nke ọkọlọtọ ahụ. E nwetara usoro nhazi oke molekul dị iche iche na nha ha site na ịkọpụta logarithm nke oke molekul dị iche iche megide oge njide ma ọ bụ site na nlọghachi ahịrị.
4.3 Ọgwụgwọ ihe nlele
Tụọ 10mg nke ihe nlele ahụ nke ọma n'ime karama volumetric 10mL, tinye obere usoro mpụtagharị, nke na-ama jijiji site na ultrasonic ruo nkeji iri, ka ihe nlele ahụ wee gbazee nke ọma ma gwakọta ya, gwakọta ya na usoro mpụtagharị ruo nha, wee tee ya site na akpụkpọ ahụ organic nke nwere oghere nke 0.2μm ~ 0.5μm, wee nyochaa nzacha ahụ dịka ọnọdụ chromatographic dị na A.4.1 si dị.
- 5. Ngụkọta nke nkesa oke molekụla metụtara
- Mgbe a tụlechara ihe ngwọta ihe nlele nke e mere na 4.3 n'okpuru ọnọdụ chromatographic nke 4.1, enwere ike nweta oke molekul dị n'ihe nlele ahụ na oke nkesa ya site na iji data chromatographic nke ihe nlele ahụ mee ihe na usoro nhazi 4.2 na ngwanrọ nhazi data GPC. Enwere ike ịgbakọ nkesa nke oke molekul dị n'etiti peptides dị iche iche site na usoro nhazi mpaghara kacha elu, dịka usoro si dị: X=A/A ngụkọta ×100
- N'ime usoro a: X - Oke oke nke peptide oke molekul dị na mkpokọta peptide dị na sample, %;
- A - Mpaghara kachasị elu nke peptide oke molekul dị n'otu akụkụ;
- Ngụkọta A - ngụkọta nke mpaghara elu nke peptide oke molekul ọ bụla, nke a gbakọrọ gaa n'otu ebe decimal.
- 6 Imeghachi ihe
- Ọdịiche zuru oke dị n'etiti mkpebi abụọ nọọrọ onwe ha e nwetara n'okpuru ọnọdụ nke imeghachi ihe agaghị agafe 15% nke nkezi mgbakọ na mwepụ nke mkpebi abụọ ahụ.
- Mgbakwunye B: Ụzọ maka ikpebi nke amino acid efu
- Nkwenye nke ọkọlọtọ: Q/320205 KAVN05-2016
- 1.2 Ihe ndị na-eme ka ihe dịghachi ndụ na ihe ndị ọzọ
- Glacial acetic acid: dị ọcha n'ihe gbasara nyocha
- Asịd Perchloric: 0.0500 mol/L
- Ihe ngosi: 0.1% ihe ngosi kristal violet (asịd glacial acetic)
- 2. Nchọpụta nke amino asịd efu
A kpọrọ nkụ ihe nlele ndị ahụ na okpomọkụ nke 80°C ruo otu awa.
Debe ihe nlele ahụ n'ime akpa kpọrọ nkụ ka o wee jụọ oyi n'ụzọ ebumpụta ụwa ruo n'okpomọkụ ụlọ ma ọ bụ jụọ oyi ruo n'okpomọkụ a na-eji eme ihe.Tụ ihe dị ka 0.1 g nke ihe nlele (nke ziri ezi ruo 0.001 g) n'ime karama kọnịk akọrọ nke 250 mL.Gaa n'ihu ngwa ngwa gaa na nzọụkwụ ọzọ iji zere ihe nlele ahụ ịmịkọrọ mmiri gburugburu ebe obibi.Tinye 25 mL nke glacial acetic acid ma gwakọta ya nke ọma ruo ihe karịrị nkeji ise.Tinye ntapụ abụọ nke ihe ngosi kristal violetTinye ihe ngwọta titration nke perchloric acid nke 0.0500 mol / L (±0.001) nke a na-akpọ titration ruo mgbe ihe ngwọta ahụ gbanwere site na odo odo ruo na njedebe ya.
Dekọọ olu nke ihe ngwọta nkịtị e riri.
- Mee ule ahụ n'otu oge ahụ.
- 3. Ngụkọta na nsonaazụ
- A na-egosipụta ọdịnaya amino acid efu X dị na reagent dị ka akụkụ oke (%) ma a na-agbakọ ya dịka usoro si dị: X = C × (V1-V0) × 0.1445/M × 100%, na usoro tne:
- C - Ntinye nke ihe ngwọta perchloric acid ọkọlọtọ na moles kwa lita (mol/L)
- V1 - Olu e ji mee titration nke ihe nlele nwere ngwọta perchloric acid nkịtị, na milliliters (mL).
- Vo - Olu eji eme titration efu na ngwọta perchloric acid nkịtị, na milliliters (mL);
M - Ibu nke ihe nlele ahụ, na gram (g).
| 0.1445: Nkezi oke amino acid nke hà nhata 1.00 mL nke ihe ngwọta perchloric acid ọkọlọtọ [c (HClO4) = 1.000 mol / L]. | 4.2.3 Ihe ngwọta titration ọkọlọtọ nke Cerium sulfate: njupụta c [Ce (SO4) 2] = 0.1 mol/L, nke a kwadebere dịka GB/T601 si dị. | |
| Nkwenye nke ụkpụrụ: Q/70920556 71-2024 | 1. Ụkpụrụ mkpebi (Fe dị ka ihe atụ) | Ihe ndị mejupụtara ígwè amino acid nwere obere mgbaze na ethanol anhydrous, a na-agbazekwa ion metal n'efu na ethanol anhydrous, e jiri ọdịiche dị na mgbaze n'etiti abụọ ahụ na ethanol anhydrous chọpụta ọnụego chelation nke amino acid iron complexes. |
| N'ime usoro a: V1 - olu nke ihe ngwọta ọkọlọtọ cerium sulfate e ji mee titration nke ihe ngwọta nnwale, mL; | Ethanol nke na-anaghị esi n'ime mmiri pụta; ihe fọdụrụ bụ otu ihe ahụ dị ka nkebi nke 4.5.2 na GB/T 27983-2011. | 3. Nzọụkwụ nyocha |
| Mee nnwale abụọ n'otu oge. Tụọ 0.1g nke ihe nlele ahụ kpọrọ nkụ na 103±2℃ ruo otu awa, nke ziri ezi ruo 0.0001g, tinye 100mL nke ethanol anhydrous iji gbazee, sachaa, sachaa ihe fọdụrụ na 100mL nke ethanol anhydrous maka opekata mpe ugboro atọ, wee bufee ihe fọdụrụ na karama conical 250mL, tinye 10mL nke ngwọta sulfuric acid dịka nkebi nke 4.5.3 dị na GB/T27983-2011 si dị, wee mee usoro ndị a dịka nkebi nke 4.5.3 si dị "Kpoo ọkụ ka ọ gbazee wee hapụ ya ka ọ dị jụụ" na GB/T27983-2011. Mee nnwale ahụ n'otu oge ahụ. | 4. Nchọpụta nke ọdịnaya ígwè zuru oke | 4.1 Ụkpụrụ nke mkpebi bụ otu ihe ahụ dị ka nkebi nke 4.4.1 dị na GB/T 21996-2008. |
4.2. Ihe ndị na-eme ka ihe na-agbanwe agbanwe na ngwọta ya
| 4.2.1 Asịd agwakọtara: Tinye 150mL nke sulfuric acid na 150mL nke phosphoric acid na 700mL mmiri wee gwakọta ya nke ọma. | 4.2.2 Ngwọta ihe ngosi Sodium diphenylamine sulfonate: 5g/L, nke a kwadebere dịka GB/T603 si dị. | 4.2.3 Ihe ngwọta titration ọkọlọtọ nke Cerium sulfate: njupụta c [Ce (SO4) 2] = 0.1 mol/L, nke a kwadebere dịka GB/T601 si dị. | |
| 4.3 Nzọụkwụ nyocha | Mee nnwale abụọ n'otu oge. Tụọ 0.1g nke ihe nlele, nke ziri ezi ruo 020001g, tinye ya n'ime karama conical 250mL, tinye 10mL nke acid agwakọtara, mgbe agbazechara ya, tinye 30ml nke mmiri na ntapụ anọ nke sodium dianiline sulfonate indicator solution, wee mee usoro ndị a dịka nkebi nke 4.4.2 si dị na GB/T21996-2008. Mee nnwale ahụ n'otu oge ahụ. | 4.4 Nnọchianya nke nsonaazụ | A gbakọrọ mkpokọta ọdịnaya ígwè X1 nke amino acid iron complexes n'ihe gbasara oke nke iron, uru egosiri na %, dịka usoro (1) si dị: |
| X1=(V-V0)×C×M×10-3×100 | V0 - ihe ngwọta ọkọlọtọ nke cerium sulfate a na-eri maka titration nke ihe ngwọta efu, mL; | V0 - ihe ngwọta ọkọlọtọ nke cerium sulfate a na-eri maka titration nke ihe ngwọta efu, mL; | C - Ngụkọta nke ihe ngwọta ọkọlọtọ cerium sulfate n'ezie, mol/L5. Ngụkọta nke ọdịnaya ígwè na chelatesA gbakọrọ ihe dị n'ime ígwè X2 dị na chelate n'ihe gbasara oke nke ígwè, uru e gosipụtara na %, dịka usoro si dị: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| N'ime usoro a: V1 - olu nke ihe ngwọta ọkọlọtọ cerium sulfate e ji mee titration nke ihe ngwọta nnwale, mL; | V2 - ihe ngwọta ọkọlọtọ cerium sulfate a na-eri maka titration nke ihe ngwọta efu, mL;nom1-Nha nke ihe nlele ahụ, g. Were nkezi mgbakọ na mwepụ nke nsonaazụ mkpebi yiri ya dị ka ihe si na mkpebi ahụ pụta, ọdịiche zuru oke nke nsonaazụ mkpebi yiri ya abụghị ihe karịrị 0.3%. | 0.05585 - oke ígwè ferrous nke a na-egosipụta na gram nke ruru 1.00 mL nke ihe ngwọta ọkọlọtọ cerium sulfate C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Nha nke ihe nlele ahụ, g. Were nkezi mgbakọ na mwepụ nke nsonaazụ mkpebi yiri ya dị ka ihe si na mkpebi ahụ pụta, ọdịiche zuru oke nke nsonaazụ mkpebi yiri ya abụghị ihe karịrị 0.3%. | 6. Ngụkọta nke ọnụego chelationỌnụego chelation X3, uru e gosipụtara na %, X3 = X2/X1 × 100Mgbakwunye C: Ụzọ maka ikpebi ọnụego chelation nke Zinpro |
Nkwenye nke ọkọlọtọ: Q/320205 KAVNO7-2016
1. Ihe ndị na-eme ka ihe dịghachi ndụ na ihe ndị ọzọ
a) Asịd Glacial acetic: dị ọcha n'ihe gbasara nyocha; b) Asịd Perchloric: 0.0500mol/L; c) Ihe ngosi: 0.1% ihe ngosi kristal violet (asịd glacial acetic)
2. Nchọpụta nke amino asịd efu
2.1 A kpọrọ nkụ ihe nlele ndị ahụ na okpomọkụ 80°C ruo otu awa.
2.2 Tinye ihe nlele ahụ n'ime akpa kpọrọ nkụ ka ọ jụọ oyi n'ụzọ ebumpụta ụwa ruo okpomọkụ ụlọ ma ọ bụ jụọ oyi ruo okpomọkụ a na-eji.
2.3 Tụọ ihe dị ka 0.1 g nke ihe nlele (nke ziri ezi ruo 0.001 g) n'ime karama kọnịk akọrọ nke 250 mL
2.4 Gaa n'ihu ngwa ngwa gaa na nzọụkwụ ọzọ iji zere ihe nlele ahụ ịmịkọrọ mmiri gburugburu.
2.5 Tinye 25mL nke glacial acetic acid ma gwakọta ya nke ọma ruo ihe karịrị nkeji ise.
2.6 Tinye ntapụ abụọ nke ihe ngosi kristal violet.
2.7 Jiri 0.0500mol/L (±0.001) nke perchloric acid mee ka ọ dị nro ruo mgbe ihe ngwọta ahụ ga-agbanwe site na odo odo gaa na akwụkwọ ndụ akwụkwọ ndụ ruo afọ 15 na-agbanweghị agba dị ka ebe njedebe.
2.8 Dekọọ olu nke ihe ngwọta nkịtị e riri.
2.9 Mee ule efu ahụ n'otu oge ahụ.
- 3. Ngụkọta na nsonaazụ
- Ndị Catalan
- Physicochemical parameters
V1 - Olu e ji mee titration nke ihe nlele nwere ngwọta perchloric acid nkịtị, na milliliters (mL).
Vo - Olu eji eme titration efu na ngwọta perchloric acid nkịtị, na milliliters (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Adreesị: No.147 Qingpu Road, Shouan Town, Pujiang County, Chengdu City, Sichuan Province, China
Ekwentị: 86-18880477902
Ngwaahịa
Mineral ndị na-adịghị ahụkebe
- Ihe ndị dị na mineral organic
- Swahili
- Ọrụ ahaziri ahazi
- Njikọ ngwa ngwa
Nkọwapụta Ụlọ ọrụ
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Pịa maka ajụjụ | © Nwebiisinka - 2010-2025: Ikike niile echekwabara. | Maapụ saịtị NCHỌTA KACHASỊ MMA Ekwentị |
| Tel | 86-18880477902 | Asụsụ Javan | Ozi ịntanetị |
| 8618880477902 | Ndị Chaịna | French | |
| Bird | Ndị Chaịna | French | Asụsụ Jamanị Spanish |
| Aquatic animals | ndị Japan | Ndị Korea | Asụsụ Arabic Grik |
| Tọki | Ịtali | ||
| Ruminant animal g/head day | January 0.75 | Indonesịa Ndị Afrịka Swidish |
Ndị Poland
- Asụsụ Bask
- Ndị Catalan
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Onye Bọlgarian
- Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Croatia
Ndị Dutch
| Application object | Urdu Ndị Vietnamese | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | onye Haiti | Ndị Hausa | Ndị Kinyarwanda Hmong Ndị Hungaria |
| Piglets and fattening pigs | Igbo | Asụsụ Javan | Kanada Ndị Khmer Kurdish |
| Ndị Kyrgyz | Asụsụ Latịn | ||
| Bird | 300～400 | 45～60 | Onye Masedonia Asụsụ Malay Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Ndị Norway
- Pashtọ
- Appearance: brownish-yellow granules
- Physicochemical parameters
Ndị Serbia
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahili
Ndị Tajik
Tamil
Telugu
Asụsụ Taịland
| Application object | Urdu Ndị Vietnamese | Content in full-value feed (mg/kg) | Efficacy |
| Ndị Yiddish | Ndi Yoruba | Zulu | Ndị Kinyarwanda Oriya Ndị Turkmen |
| Uighur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
